A recent morphological description of monodon baculovirus might correspond to this virus 47. Ultrastructural and pathogenesis of monodon baculovirus in. Monodon baculovirus mbv infects epithelia1 cells of the hepatopancreas hp and midgut of penaeus monodon and other penaeid shrimp. The effect of viral infection on the relationship between. Prevalence of monodon baculovirus mbv in wild shrimp penaeus.
Monodon baculovirus mbv forms polyhedral occlusion bodies obs. Even though mbv is not a severe pathogen for the black tiger prawn penaeus monodon, it should be eliminated from the farming system because it is unlikely that. We report the use of recombinant baculovirus mediated transduction using hybrid promoter system for transgene expression in lymphoid cells of penaeus monodon. Monodon baculovirus mbv disease viral infections in penaeid shrimps affected shrimps exhibit palebluishgray to dark blueblack coloration, sluggish and inactive swimming movements, loss of. Wssv, yellow head virus yhv, monodon baculovirus mbv and to a lesser extent, luminescent bacterial infections. Mbv monodon baculovirus the occlusion bodies ob formed as monodon baculovirus mbv replicate are easily visible using a microscope. More than 600 recombinant genes have been expressed in baculoviruses to date. However, hznv2 lacks several key genetic features of baculoviruses including the late transcriptional regulation factor, lef1. Decontamination of monodon baculovirus in marine shrimp. Multiple viral pathogens occurrence in tiger shrimp. Diseases such as whitespot baculovirus and yellowhead virus have led to a great. In hatcheries, bp and bmn often cause serious epizootics in the larval and early. Occurrence of viral pathogens in penaeus monodon postlarvae from aquaculture hatcheries toms c.
The present study attempts to investigate the effect of penaeus monodon. Penaeus monodon mature and breed only in tropical marine habitats and spend their larval, juvenile, adolescent and subadult stages in coastal estuaries, lagoons or mangrove areas. The israeli journal of aquaculture bamidgeh 6, 2009 fig. Necrosois virus, monodon baculovirus, white spot syndrome virus. Evidence for the presence of white spot syndrome virus. The genome and occlusion bodies of marine penaeus monodon. Pdf prevalence of white spot syndrome virus and monodon. Analysis of the genome of the sexually transmitted insect. Environmental assessment report import of specific. White spot baculovirus syndrome in the indian shrimp. Baculovirus infections may result in disease in cultured penaeids that is accompanied by high mortality rates. Millions of metric tons dominated by penaeus monodon. The present invention relates to the establishment of an immortal shrimp cell line pmo derived from penaeus monodon. There appear to be more ob when pl are stressed, so.
World production of shrimp from capture fisheries and aquaculture. The simultaneous presence of monodon baculovirus mbv and whitespot syndrome virus wssv in apparently healthy postlarvae of penaeus monodon from different hatcheries in india was studied by. Focusing on the giant tiger shrimp penaeus monodon. Among the viruses infecting penaeid shrimp, monodon type baculovirus mbv otherwise known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv, is one of the widely reported and well described viruses. Monodon baculovirus mbv is an economically important and one of the. Cell membranes can also blend or merge without fusion of the cellular contents. Production was dominated by penaeus monodonuntil 2002 and by penaeus vannamei thereafter.
Ancient coevolution of baculoviruses and their insect hosts. Another less accurate process, microhomology mediated end joining is also used to. Occurrence of viral pathogens in penaeus monodon post. The open access israeli journal of aquaculture bamidgeh. Poor quality seed, coupled with the slow growth of p.
Prevalence of white spot syndrome virus and monodon baculovirus in penaeus monodon broodstock and postlarvae from hatcheries in southeast coast of india. The multiplex pcr master mix comprised of 2 l of 10x primer mix. Prevalence of white spot syndrome virus wssv and monodon baculovirus mbv infection in penaeus monodon postlarvae in vietnam. This cell line is susceptible to and capable of mass producing virus, which includes, but not limited to, white spot syndrome virus wssv, panaeus monodon baculovirus. Penaeus monodon type baculovirus tetrahedral baculovirosis baculovirus penaei taura syndrome white spot disease white tail disease yellowhead disease. Colourimetric pcrbased detection of monodon baculovirus. Monodon baculovirus mbv is a member of nucleopolyhedrosis virus npv group that infect penaeid shrimps. Two years later, it was designated monodon baculovirus mbv 2, and this name is still commonly used today. Pdf prevalence of white spot syndrome virus wssv and. The pcr was shown to be specific for mbv dna by not amplifying prawn dna or dna preparations of baculovirus penaei bp, white spot baculovirus wsbv, bennettae baculovirus and insect. A survey on the presence of the viruses of two economically significant diseases, white spot syndrome virus wssv and monodon baculovirus mbv in wild. Decontamination of monodon baculovirus in marine shrimp eggs using. Another less accurate process, microhomologymediated end joining is also used to.
Decontamination of monodon baculovirus in marine shrimp eggs using upwelling flowthrough system article pdf available july 2015 with 110 reads how we measure reads. Manual of histological staining methods of the armed forces. Pdf penaeus monodon type baculovirus mbv is a horizontal transmission virus disease especially found in marine shrimp. Mbv virions are rodshaped, nonoccluded or occluded and enveloped within a paracrystalline occlusion body. The objective of our study was to explain the histopathological changes of monodon baculovirus mbv in hepatopancreas and midgut tissues of the cultured penaeus vannamei. Current status of viral diseases in asian shrimp aquaculture. Detection of monodon baculovirus and whitespot syndrome. In the wild, they show marked nocturnal activity, burrowing into bottom substratum during the day and emerging at night to search for food as benthic feeders. Among the viruses infecting penaeid shrimp, monodon type baculovirus mbv otherwise known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv, is one of the widely. A record and prevalence of monodon baculovirus from. The trees were found by a heuristic search starting with neighbor joining and nearestneighbor.
Shrimp, penaeus semisulcatus, penaeus monodon baculovirus, histopathology. Penaeus monodon, commonly known as the giant tiger prawn or asian tiger shrimp is a marine. Adw doesnt cover all species in the world, nor does it include all the latest scientific. Attempts at producing a hybridised penaeus mondon cell line by cellular fusion. Guide to baculovirus expression vector systems bevs and. Occurrence of viral pathogens in penaeus monodon postlarvae. Penaeus monodontype baculovirus mbv is found in many parts of the world from.
Us6143547a cell line derived from penaeus monodon and a. Request pdf prevalence of monodon baculovirus mbv in wild shrimp. The cytopathology, virogenesis and replication of monodon baculovirus mbv in penaeus monodon from australia are described. Onfarm feed management practices for black tiger shrimp. Since 1985, when the first protein il2 was produced in large scale from a recombinant baculovirus. Monodon baculovirus mbv mbv is the first reported virus of p. Penaeus monodon baculovirus mbv is a rodshaped virus possessing a. Two years later, it was designated monodon baculovirus mbv 2. Pdf decontamination of monodon baculovirus in marine. Among the viruses infecting penaeid shrimp, monodon type baculovirus mbv otherwise known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv, is one of the widely reported. Major viral diseases of penaeus monodon in taiwan chufang lo, yunshiang chang, shoen peng and guanghsiung kou received, march 10, 2003.
It is a nuclear polyhedrosis virus npv of the family. The pcr reactions were done as described in oie manual of diagnosis for aquatic animals 28. Monodon baculovirus mbv disease viral infections in. Mbv virions are rodshaped, nonoccluded or occluded. Polymerase chain reaction pcr assays have been reported for specific and accurate. Penaeus monodon baculovirus variously known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv or monodon baculovirus mbv, the causative agent of spherical baculovirosis in shrimp p. Freepenaeus monodon hatchery manual based on the experience in india. Decontamination, monodon baculovirus, mbv, shrimp egg, upwelling flowthrough system. White spot baculovirus syndrome in the indian shrimp penaeus monodon and p.
Improvement of pcr method for the detection of monodon. Monodon baculovirus has been known to be invasive in 85100% of the shrimp hatcheries, in early or late stages of shrimp. Spherical baculovirosis penaeus monodon type baculovirus p. Several investigators have demonstrated that prawn primary cell cultures can support in vitro growth of monodon baculovirus. The animal diversity web is an educational resource written largely by and for college students. Penaeus monodon is the secondmost widely cultured prawn species in the world, after only whiteleg shrimp, litopenaeus vannamei. Request pdf improvement of pcr method for the detection of monodon baculovirus mbv in penaeid shrimp penaeus monodontype baculovirus mbv.